The multiplication of undifferentiated cells is a propagation-limited process, characterized by distinct growth stages: an early stage, followed by an optimum mid-range stage, and eventually a stage where growth and recovery of viable germinates decline. This development typically follows a normal curve pattern.
Advanced techniques have been developed to culture mature embryos and regenerate undifferentiated callus tissues. This process not only provides an additional tool for our germplasm preservation efforts but also reinvigorates cell lines to enhance the proliferation of undifferentiated cells.
This method successfully saved line 345 from complete loss, and other lines have been able to regenerate callus tissues for cryopreservation and additional material for new embryo production.